Die Akkumulation von Laktat im Rahmen der Wundheilung stimuliert die Expression von Schlüsselgenen für das Homing mesenchymaler Stammzellen.

Introduction: Surgical wounds are characterized by tissue lactate accumulation. A recent study was able to detect an increased stem cell count in experimental wounds treated with exogenous lactate. Nevertheless, it remains unclear whether these cells are locally activated stem cells or migrated stem cells from the blood circulation. Therefore, the aim of this study was to investigate the effect of lactate on genes involved in stem cell homing. Material and Methods: Human umbilical vein endothelial cells (HUVEC) and mesenchymal stem cells (MSC) were incubated with different concentrations of lactate. Gene expression analysis was conducted using oligonucleotid-microarrays. Differentially expressed genes of interest were additionally evaluated using qRT-PCR. Protein analysis was performed using FACS, Westernblot and ELISA. Results are given as mean ± SD or as fold-change to control. Results: Lactate induced gene expression of c-Kit (2,4-fold) and CXCR-4 (2,2-fold) in MSC. In HUVEC, gene expression of CXCL-12 (SDF-1) and SCF was enhanced. In lactate-stimulated MSC, FACS analysis confirmed a higher expression for c-Kit and westernblotting for CXCR-4. In the supernatant of lactatetreated HUVEC, ELISA detected higher concentrations of CXCL-12 (SDF-1) and SCF compared to their corresponding controls. Conclusion: Lactate enhances the expression of genes involved in mesenchymal stem cell homing. The association between lactate and stem cell homing provides new insights into the wound healing physiology. Hence, this data might serve for new treatment strategies. [ABSTRACT FROM AUTHOR]