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Quercetin regulates organic ion transporter and uromodulin expression and improves renal function in hyperuricemic mice.
- Source :
- European Journal of Nutrition; Aug2012, Vol. 51 Issue 5, p593-606, 14p
- Publication Year :
- 2012
-
Abstract
- Background: Renal organic ion transporters and uromodulin (UMOD) play the important roles in renal urate excretion and function. Hyperuricemia is considered as a risk factor for the development of renal dysfunction. The flavonoid quercetin in diets exerts the hypouricemic and nephroprotective effects. Purposes: To evaluate the effects of quercetin on renal organic ion transporters and UMOD in hyperuricemic mice. Methods: Kun-Ming mice were divided into normal and hyperuricemic groups receiving water, 25, 50 and 100 mg/kg quercetin, 5 mg/kg allopurinol, respectively. Hyperuricemic mice were orally gavaged with 250 mg/kg oxonate daily for 1 week. Quercetin and allopurinol were orally gavaged on the day when oxonate or water was given 1 h later. After 1 week, serum uric acid, creatinine and blood urea nitrogen concentrations, excretion of urate and creatinine, and fractional excretion of uric acid were measured. The mRNA and protein levels of renal urate transporter 1 (mURAT1), glucose transporter 9 (mGLUT9), organic anion transporter 1 (mOAT1) and organic cation/carnitine transporters (mOCT1, mOCT2, mOCTN1 and mOCTN2) in mice were analyzed. Simultaneously, UMOD levels in serum, urine and kidney, as well as renal UMOD mRNA expression were detected. Results: Quercetin significantly restored oxonate-induced abnormalities of these biochemical indexes compared with normal vehicle group. Furthermore, it remarkably prevented expression changes of renal organic ion transporters and UMOD, and UMOD level alteration in hyperuricemic mice. Conclusions: These results suggest that quercetin has the uricosuric and nephroprotective actions mediated by regulating the expression levels of renal organic ion transporters and UMOD. [ABSTRACT FROM AUTHOR]
- Subjects :
- NEPHROTOXICOLOGY
ANALYSIS of variance
ANIMAL experimentation
BIOPHYSICS
COMPARATIVE studies
CREATININE
DOSE-effect relationship in pharmacology
HISTOLOGICAL techniques
KIDNEYS
RESEARCH methodology
MICE
POLYMERASE chain reaction
QUERCETIN
RESEARCH funding
STATISTICS
URIC acid
URINALYSIS
WESTERN immunoblotting
DATA analysis
REVERSE transcriptase polymerase chain reaction
MEMBRANE glycoproteins
ION transport (Biology)
DESCRIPTIVE statistics
BLOOD urea nitrogen
PREVENTION
Subjects
Details
- Language :
- English
- ISSN :
- 14366207
- Volume :
- 51
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- European Journal of Nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 77735110
- Full Text :
- https://doi.org/10.1007/s00394-011-0243-y