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Conditional ablation of CD205+ conventional dendritic cells impacts the regulation of T-cell immunity and homeostasis in vivo.
- Source :
- Proceedings of the National Academy of Sciences of the United States of America; 7/10/2012, Vol. 109 Issue 28, p11288-11293, 6p
- Publication Year :
- 2012
-
Abstract
- Dendritic cells (DCs) are composed of multiple subsets that play a dual role in inducing immunity and tolerance. However, it is unclear how CD205<superscript>+</superscript> conventional DCs (cDCs) control immune responses in vivo. Here we generated knock-in mice with the selective conditional ablation of CD205<superscript>+</superscript> cDCs. CD205+ cDCs contributed to antigen-specific priming of CD4<superscript>+</superscript> T cells under steady-state conditions, whereas they were dispensable for antigen-specific CD4<superscript>+</superscript> T-cell responses under inflammatory conditions. In contrast, CD205<superscript>+</superscript> cDCs were required for antigen-specific priming of CD8<superscript>+</superscript> T cells to generate cytotoxic T lymphocytes (CTLs) mediated through cross-presentation. Although CD205<superscript>+</superscript> cDCs were involved in the thymic generation of CD4<superscript>+</superscript> regulatory T cells (Tregs), they maintained the homeostasis of CD4<superscript>+</superscript> Tregs and CD4<superscript>+</superscript> effector T cells in peripheral and mucosal tissues. On the other hand, CD205<superscript>+</superscript> cDCs were involved in the inflammation triggered by Toll-like receptor ligand as well as bacterial and viral infections. Upon microbial infections, CD205<superscript>+</superscript> cDCs contributed to the cross-priming of CD8<superscript>+</superscript> T cells for generating antimicrobial CTLs to efficiently eliminate pathogens, whereas they suppressed antimicrobial CD4<superscript>+</superscript> T-cell responses. Thus, these findings reveal a critical role for CD205<superscript>+</superscript> cDCs in the regulation of T-cell immunity and homeostasis in vivo. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 109
- Issue :
- 28
- Database :
- Complementary Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 78108510
- Full Text :
- https://doi.org/10.1073/pnas.1202208109