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Protective effects of Lycium barbarum polysaccharide on neonatal rat primary cultured hippocampal neurons injured by oxygen-glucose deprivation and reperfusion.
- Source :
- Journal of Molecular Histology; Oct2012, Vol. 43 Issue 5, p535-542, 8p
- Publication Year :
- 2012
-
Abstract
- This study investigated the protective effects of Lycium barbarum polysaccharide (LBP) on alleviating injury from oxygen-glucose deprivation/reperfusion (OGD/RP) in primary cultured rat hippocampal neurons. Cultured hippocampal neurons were exposed to oxygen-glucose deprivation (OGD) for 2 h followed by a 24 h re-oxygenation. The MTT assay and the lactate dehydrogenase (LDH) release were used to determine the neuron viability. Superoxide dismutase (SOD), Glutathione peroxidase (GSH-PX), malondialdehyde (MDA) were determined by spectrophotometry using commercial kits. Mitochondrial membrane potential (MMP) and the intracellular free calcium concentration ([Ca]) in hippocampal neurons were measured using the confocal laser scanning microscope (CLSM). Treatment with LBP (10-40 mg/l) significantly attenuated neuronal damage and inhibited LDH release in a dose-dependent manner. Furthermore, LBP enhanced activities of SOD and GSH-PX but it decreased their MDA content, inhibited [Ca] elevation and decrease of MMP in ischemia-reperfusion treated hippocampal neurons. These findings suggested that LBP may be a potential neuroprotective agent for cerebral ischemia-reperfusion injury. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15672379
- Volume :
- 43
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Journal of Molecular Histology
- Publication Type :
- Academic Journal
- Accession number :
- 80730083
- Full Text :
- https://doi.org/10.1007/s10735-012-9420-4