Molecular Basis of Tobacco-Induced Bacterial Biofilms: An In Vitro Study.

The article presents a study which evaluates the expression of biofilm-related genes after exposure to oxidative stress and tobacco smoke. The study uses crystal violet straining to measure bacterial biofilm mass and optical density measurement. Results show that repetitive exposure to tobacco smoke leads to molecular changes in genes and oxidative stress exposure in H₂O₂ form produces biofilm growth.