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ST13, a proliferation regulator, inhibits growth and migration of colorectal cancer cell lines.
- Source :
- Journal of Zhejiang University: Science B; Nov2012, Vol. 13 Issue 11, p884-893, 10p
- Publication Year :
- 2012
-
Abstract
- Background and objective: ST13, is the gene encoding the HSP70 interacting protein (HIP). Previous research has shown that ST13 mRNA and protein levels are down-regulated in colorectal cancer (CRC) tissues compared with adjacent normal tissues. This study aims at the role of ST13 in the proliferation and migration of CRC cells. Methods: The transcript level of ST13 in different CRC cell lines was evaluated by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). ST13-overexpressed and ST13-knockdown CRC cells were constructed respectively by lentiviral transduction, followed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay, plate colony formation, cell-cycle analysis, and migration assays to evaluate the influence of ST13 on proliferation and migration in vitro. Moreover, a mouse xenograft study was performed to test in vivo tumorigenicity of ST13-knockdown CRC cells. Results: Lentivirus-mediated overexpression of ST13 in CRC cells inhibited cell proliferation, colony formation, and cell migration in vitro. In contrast, down-regulation of ST13 by lentiviral-based short hairpin RNA (shRNA) interference in CRC cells significantly increased cell proliferation and cloning efficiency in vitro. In addition, down-regulation of ST13 expression significantly increased the tumorigenicity of CRC cells in vivo. Conclusions: ST13 gene is a proliferation regulator that inhibits tumor growth in CRC and may affect cell migration. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16731581
- Volume :
- 13
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Journal of Zhejiang University: Science B
- Publication Type :
- Academic Journal
- Accession number :
- 83225089
- Full Text :
- https://doi.org/10.1631/jzus.B1200037