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Proteomic Characterization of a Mouse Model of Familial Danish Dementia.
- Source :
- Journal of Biomedicine & Biotechnology; 2012, Vol. 2012, p1-8, 8p
- Publication Year :
- 2012
-
Abstract
- A dominant mutation in the ITM2B/BRI2 gene causes familial Danish dementia (FDD) in humans. To model FDD in animal systems, a knock-in approach was recently implemented in mice expressing a wild-type and mutant allele, which bears the FDD-associated mutation. Since these FDD<subscript>KI</subscript> mice show behavioural alterations and impaired synaptic function, we characterized their synaptosomal proteome via two-dimensional differential in-gel electrophoresis. After identification by nanoliquid chromatography coupled to electrospray-linear ion trap tandem mass spectrometry, the differentially expressed proteins were classified according to their gene ontology descriptions and their predicted functional interactions. The Dlg4/Psd95 scaffold protein and additional signalling proteins, including protein phosphatases, were revealed by STRING analysis as potential players in the altered synaptic function of FDD<subscript>KI</subscript> mice. Immunoblotting analysis finally demonstrated the actual downregulation of the synaptosomal scaffold protein Dlg4/Psd95 and of the dual-specificity phosphatase Dusp3 in the synaptosomes of FDD<subscript>KI</subscript> mice. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 11107243
- Volume :
- 2012
- Database :
- Complementary Index
- Journal :
- Journal of Biomedicine & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 85115349
- Full Text :
- https://doi.org/10.1155/2012/728178