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Xenogenic Esophagus Scaffolds Fixed with Several Agents: Comparative In Vivo Study of Rejection and Inflammation.
- Source :
- Journal of Biomedicine & Biotechnology; 2012, Vol. 2012, p1-11, 11p
- Publication Year :
- 2012
-
Abstract
- Most infants with long-gap esophageal atresia receive an esophageal replacement with tissue from stomach or colon, because the native esophagus is too short for true primary repair. Tissue-engineered esophageal conducts could present an attractive alternative. In this paper, circular decellularized porcine esophageal scaffold tissues were implanted subcutaneously into Sprague-Dawley rats. Depending on scaffold cross-linking with genipin, glutaraldehyde, and carbodiimide (untreated scaffolds : positive control; bovine pericardium : gold standard), the number of infiltrating fibroblasts, lymphocytes, macrophages, giant cells, and capillaries was determined to quantify the host response after 1, 9, and 30 days. Decellularized esophagus scaffolds were shown to maintain native matrix morphology and extracellular matrix composition. Typical inflammatory reactions were observed in all implants; however, the cellular infiltration was reduced in the genipin group. We conclude that genipin is the most efficient and best tolerated crosslinking agent to attenuate inflammation and to improve the integration of esophageal scaffolds into its surrounding tissue after implantation. [ABSTRACT FROM AUTHOR]
- Subjects :
- ANALYSIS of variance
ANIMAL experimentation
ANTIGENS
BIOPHYSICS
CHEMICAL reagents
CONFIDENCE intervals
CULTURE media (Biology)
ESOPHAGUS
GRAFT rejection
HETEROCYCLIC compounds
RESEARCH methodology
RATS
RESEARCH funding
STATISTICS
SWINE
U-statistics
XENOGRAFTS
DATA analysis
DESCRIPTIVE statistics
CHILDREN
Subjects
Details
- Language :
- English
- ISSN :
- 11107243
- Volume :
- 2012
- Database :
- Complementary Index
- Journal :
- Journal of Biomedicine & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 85115417
- Full Text :
- https://doi.org/10.1155/2012/948320