Back to Search
Start Over
Photo(chemo)therapy Reduces Circulating Th17 Cells and Restores Circulating Regulatory T Cells in Psoriasis.
- Source :
- PLoS ONE; Jan2013, Vol. 8 Issue 1, Special section p1-9, 9p
- Publication Year :
- 2013
-
Abstract
- Background: Photo(chemo)therapy is widely used to treat psoriasis, the pathogenesis of which might be caused by an imbalance of Th17 cells/regulatory T cells (Treg). In the present study, we evaluated the effects of photo(chemo)therapy on the Th17/Treg balance and Treg function. Methods: Peripheral blood was obtained from psoriasis patients treated with bath-psoralen ultraviolet A (UVA, n = 50) or narrowband ultraviolet B (UVB, n = 18), and age-matched healthy volunteers (n = 20). CD3<superscript>+</superscript> CD4<superscript>+</superscript>IL-17A<superscript>+</superscript> or CD4<superscript>+</superscript> CD25<superscript>+</superscript> Foxp3<superscript>+</superscript> cells were analyzed to estimate Th17 or Treg number by fluorescence-activated cell sorting. Moreover,CD4<superscript>+</superscript> CD25<superscript>+</superscript>T cells from patients treated with PUVA(n = 14) were incubated in CFSE and activated with or without CD4<superscript>+</superscript>CD25<superscript>+</superscript>T cells, and the suppressive function of CD4<superscript>+</superscript>CD25<superscript>+</superscript>T cells were analyzed. Results: Photo(chemo)therapy significantly reduced Th17 levels from 5.6663.15% to 2.9662.89% in patients with increased Th17 (Th17/CD4.3.01% [mean+SD of controls]). In contrast, photo(chemo)therapy significantly increased Treg levels from 2.77‡0.75 to 3.40‡1.88% in patients with less than 4.07% Treg level, defined as the mean of controls. Furthermore, while Treg suppressed the CD4<superscript>+</superscript>CD25<superscript>-</superscript> T cell proliferation to a greater extent in controls (Treg Functional Ratio 94.4‡4.28%) than in patients (70.3‡25.1%), PUVA significantly increased Treg Functional Ratio to 88.1‡6.47%. Th17 levels in severe patients (>30 PASI) were significantly higher as compared to controls. Th17 levels that were left after treatment in the patients not achieving PASI 50 (3.78‡4.18%) were significantly higher than those in the patients achieving PASI 75 (1.83‡1.87%). Treg levels in patients achieving PASI 90 (4.89‡1.70%) were significantly higher than those in the patients not achieving PASI 90 (3.90‡1.66%). Treg levels prior to treatment with Th17 high decreased group (5.16‡2.20%) was significantly higher than that with Th17 high increased group (3.33‡1.39%). Conclusion: These findings indicate that Treg is dysfunctional in psoriasis patients, and photochemotherapy restores those dysfunctional Treg. Photo(chemo)therapy resolved the Th17/Treg imbalance in patients with psoriasis. [ABSTRACT FROM AUTHOR]
- Subjects :
- PHOTOCHEMOTHERAPY
PSORIASIS
T cells
CELL proliferation
CELLS
PATIENTS
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 85384034
- Full Text :
- https://doi.org/10.1371/journal.pone.0054895