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Overexpression of Arabidopsis YUCCA6 in Potato Results in High-Auxin Developmental Phenotypes and Enhanced Resistance to Water Deficit.

Authors :
Kim, Jeong Im
Baek, Dongwon
Park, Hyeong Cheol
Chun, Hyun Jin
Oh, Dong-Ha
Lee, Min Kyung
Cha, Joon-Yung
Kim, Woe-Yeon
Kim, Min Chul
Chung, Woo Sik
Bohnert, Hans J.
Lee, Sang Yeol
Bressan, Ray A.
Lee, Shin-Woo
Yun, Dae-Jin
Source :
Molecular Plant (Oxford University Press / USA); Mar2013, Vol. 6 Issue 2, p337-349, 13p
Publication Year :
2013

Abstract

Indole-3-acetic acid (IAA), a major plant auxin, is produced in both tryptophan-dependent and tryptophan-independent pathways. A major pathway in Arabidopsis thaliana generates IAA in two reactions from tryptophan. Step one converts tryptophan to indole-3-pyruvic acid (IPA) by tryptophan aminotransferases followed by a rate-limiting step converting IPA to IAA catalyzed by YUCCA proteins. We identified eight putative StYUC (Solanum tuberosum YUCCA) genes whose deduced amino acid sequences share 50%–70% identity with those of Arabidopsis YUCCA proteins. All include canonical, conserved YUCCA sequences: FATGY motif, FMO signature sequence, and FAD-binding and NADP-binding sequences. In addition, five genes were found with ~50% amino acid sequence identity to Arabidopsis tryptophan aminotransferases. Transgenic potato (Solanum tuberosum cv. Jowon) constitutively overexpressing Arabidopsis AtYUC6 displayed high-auxin phenotypes such as narrow downward-curled leaves, increased height, erect stature, and longevity. Transgenic potato plants overexpressing AtYUC6 showed enhanced drought tolerance based on reduced water loss. The phenotype was correlated with reduced levels of reactive oxygen species in leaves. The results suggest a functional YUCCA pathway of auxin biosynthesis in potato that may be exploited to alter plant responses to the environment. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
16742052
Volume :
6
Issue :
2
Database :
Complementary Index
Journal :
Molecular Plant (Oxford University Press / USA)
Publication Type :
Academic Journal
Accession number :
86428191
Full Text :
https://doi.org/10.1093/mp/sss100