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Both CpG Methylation and Activation-Induced Deaminase Are Required for the Fragility of the Human bcl-2 Major Breakpoint Region: Implications for the Timing of the Breaks in the t(14;18) Translocation.

Authors :
Xiaoping Cui
Zhengfei Lu
Kurosawa, Aya
Klemm, Lars
Bagshaw, Andrew T.
Tsai, Albert G.
Gemmell, Neil
Müschen, Markus
Adachi, Noritaka
Chih-Lin Hsieh
Lieber, Michael R.
Source :
Molecular & Cellular Biology; Mar2013, Vol. 33 Issue 5, p947-957, 11p
Publication Year :
2013

Abstract

The t(14;18) chromosomal translocation typically involves breakage at the bcl-2 major breakpoint region (MBR) to cause human follicular lymphoma. A theory to explain the striking propensity of the MBR breaks at three CpG clusters within the 175-bp MBR region invoked activation-induced deaminase (AID). In a test of that theory, we used here minichromosomal substrates in human pre-B cell lines. Consistent with the essential elements of the theory, we found that the MBR breakage process is indeed highly dependent on DNA methylation at the CpG sites and highly dependent on the AID enzyme to create lesions at peak locations within the MBR. Interestingly, breakage of the phosphodiester bonds at the AID-initiated MBR lesions is RAG dependent, but, unexpectedly, most are also dependent on Artemis. We found that Artemis is capable of nicking small heteroduplex structures and is even able to nick single-base mismatches. This raises the possibility that activated Artemis, derived from the unjoined D to J<subscript>H</subscript> DNA ends at the IgH locus on chromosome 14, nicks AID-generated TG mismatches at methyl CpG sites, and this would explain why the breaks at the chromosome 18 MBR occur within the same time window as those on chromosome 14. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
02707306
Volume :
33
Issue :
5
Database :
Complementary Index
Journal :
Molecular & Cellular Biology
Publication Type :
Academic Journal
Accession number :
86635594
Full Text :
https://doi.org/10.1128/MCB.01436-12