Growth Kinetics of Human Mesenchymal Stem Cells in a 3-L Single-Use, Stirred-Tank Bioreactor.

Authors :: Donghui Jing
Sunil, Neethu
Punreddy, Sandhya
Aysola, Manjula
Kehoe, Daniel
Murrel, Julie
Rook, Martha
Niss, Knut
Source :: BioPharm International; Apr2013, Vol. 26 Issue 4, p28-38, 7p
Publication Year :: 2013
Abstract: The article presents a study which describes the characteristics of growth of the human mesenchymal stem cells (hMSCs) that are cultured in stirred-tank bioreactor. Methods used include hMSCs isolation from the unprocessed and fresh bone marrow, human mesenchymal stem-cell culture, and microcarrier preparation. Study reveals the role of high lactate concentration in the growth of hMSC in the microcarriers.

Subjects :: MESENCHYMAL stem cells
STEM cell culture
BIOREACTORS
BONE marrow
LACTATES

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