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NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - protein complexes during leaf senescence.
- Source :
- Plant Journal; May2013, Vol. 74 Issue 4, p652-662, 11p, 2 Diagrams, 4 Graphs
- Publication Year :
- 2013
-
Abstract
- Yellowing/chlorophyll breakdown is a prominent phenomenon in leaf senescence, and is associated with the degradation of chlorophyll - protein complexes. From a rice mutant population generated by ionizing radiation, we isolated nyc4-1, a stay-green mutant with a defect in chlorophyll breakdown during leaf senescence. Using gene mapping, nyc4-1 was found to be linked to two chromosomal regions. We extracted Os07g0558500 as a candidate for NYC4 via gene expression microarray analysis, and concluded from further evidence that disruption of the gene by a translocation-related event causes the nyc4 phenotype. Os07g0558500 is thought to be the ortholog of THF1 in Arabidopsis thaliana. The thf1 mutant leaves show variegation in a light intensity-dependent manner. Surprisingly, the F<subscript>v</subscript>/ F<subscript>m</subscript> value remained high in nyc4-1 during the dark incubation, suggesting that photosystem II retained its function. Western blot analysis revealed that, in nyc4-1, the PSII core subunits D1 and D2 were significantly retained during leaf senescence in comparison with wild-type and other non-functional stay-green mutants, including sgr-2, a mutant of the key regulator of chlorophyll degradation SGR. The role of NYC4 in degradation of chlorophyll and chlorophyll - protein complexes during leaf senescence is discussed. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09607412
- Volume :
- 74
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Plant Journal
- Publication Type :
- Academic Journal
- Accession number :
- 87549926
- Full Text :
- https://doi.org/10.1111/tpj.12154