Back to Search
Start Over
Re-Evaluation of PD-1 Expression by T Cells as a Marker for Immune Exhaustion during SIV Infection.
- Source :
- PLoS ONE; Mar2013, Vol. 8 Issue 3, p1-10, 10p
- Publication Year :
- 2013
-
Abstract
- PD-1 expression is generally associated with exhaustion of T cells during chronic viral infections based on the finding that PD-1 expressing cells respond poorly to antigen activation and blockade of PD-1/PD-ligand interaction restores such antigen specific responses in vitro. We tested this hypothesis by examining PD-1 expression on virus-specific CD8 T cells and total T cells in vivo to determine whether PD-1 expression constitutes a reliable marker of immune exhaustion during SIV infection. The expression of PD-1 and Ki67 was monitored longitudinally on T cell subsets in peripheral blood, bone marrow, lymph node and rectal biopsy specimens from rhesus macaques prior to and post infection with pathogenic SIVmac239. During the course of infection, a progressive negative correlation was noted between PD-1 density and Ki67 expression in p11CM<superscript>+</superscript> CD8<superscript>+</superscript> T cells, as seen in other studies. However, for total and memory CD4 and CD8 T cells, a positive correlation was observed between PD-1 and Ki67 expression. Thus, while the levels of non-proliferating PD-1<superscript>+</superscript> p11CM<superscript>+</superscript> CD8 T cells were markedly elevated with progressing infection, such an increase was not seen on total T cells. In addition, total memory PD1<superscript>+</superscript> T cells exhibited higher levels of CCR5 than PD-1<superscript>−</superscript> T cells. Interestingly, few PD-1<superscript>+</superscript> CD8<superscript>+</superscript> T cells expressed CCR7 compared to PD-1<superscript>+</superscript> CD4 T cells and PD-1<superscript>−</superscript> T cells. In conclusion, overall PD1<superscript>+</superscript> T cells likely represent a particular differentiation stage or trafficking ability rather than exhaustion and in the context of chronic SIV infection, the level of PD-1 expression by T cells does not by itself serve as a reliable marker for immune exhaustion. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 87681885
- Full Text :
- https://doi.org/10.1371/journal.pone.0060186