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Effects of (--)-Gallocatechin-3-Gallate on Tetrodotoxin-Resistant Voltage-Gated Sodium Channels in Rat Dorsal Root Ganglion Neurons.
- Source :
- International Journal of Molecular Sciences; May2013, Vol. 14 Issue 5, p9779-9789, 11p, 1 Diagram, 4 Graphs
- Publication Year :
- 2013
-
Abstract
- The (--)-gallocatechin-3-gallate (GCG) concentration in some tea beverages can account for as much as 50% of the total catechins. It has been shown that catechins have analgesic properties. Voltage-gated sodium channels (Nav) mediate neuronal action potentials. Tetrodotoxin inhibits all Nav isoforms, but Nav1.8 and Nav1.9 are relatively tetrodotoxin-resistant compared to other isoforms and functionally linked to nociception. In this study, the effects of GCG on tetrodotoxin-resistant Na<superscript>+</superscript> currents were investigated in rat primary cultures of dorsal root ganglion neurons via the whole-cell patch-clamp technique. We found that 1 μM GCG reduced the amplitudes of peak current density of tetrodotoxin-resistant Na<superscript>+</superscript> currents significantly. Furthermore, the inhibition was accompanied by a depolarizing shift of the activation voltage and a hyperpolarizing shift of steady-state inactivation voltage. The percentage block of GCG (1 μM) on tetrodotoxin-resistant Na<superscript>+</superscript> current was 45.1% ± 1.1% in 10 min. In addition, GCG did not produce frequency-dependent block of tetrodotoxin-resistant Na<superscript>+</superscript> currents at stimulation frequencies of 1 Hz, 2 Hz and 5 Hz. On the basis of these findings, we propose that GCG may be a potential analgesic agent. [ABSTRACT FROM AUTHOR]
- Subjects :
- CATECHIN
GREEN tea
ANALGESICS
SODIUM channels
NEURONS
Subjects
Details
- Language :
- English
- ISSN :
- 16616596
- Volume :
- 14
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- International Journal of Molecular Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 87765186
- Full Text :
- https://doi.org/10.3390/ijms14059779