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Functional cysteine-less subunits of the transporter associated with antigen processing (TAP1 and TAP2) by de novo gene assembly
- Source :
- FEBS Letters; Jan2003, Vol. 533, p42, 5p
- Publication Year :
- 2003
-
Abstract
- Within the adaptive immune system the transporter associated with antigen processing (TAP) plays a pivotal role in loading of peptides onto major histocompatibility (MHC) class I molecules. As a central tool to investigate the structure and function of the TAP complex, we created cysteine-less human TAP subunits by de novo gene synthesis, replacing all 19 cysteines in TAP1 and TAP2. After expression in TAP-deficient human fibroblasts, cysteine-less TAP1 and TAP2 are functional with respect to adenosine triphosphate (ATP)-dependent peptide transport and inhibition by ICP47 from herpes simplex virus. Cysteine-less TAP1 and TAP2 restore maturation and intracellular trafficking of MHC class I molecules to the cell surface. [Copyright &y& Elsevier]
- Subjects :
- PEPTIDES
CHEMILUMINESCENCE
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 533
- Database :
- Complementary Index
- Journal :
- FEBS Letters
- Publication Type :
- Academic Journal
- Accession number :
- 8793310
- Full Text :
- https://doi.org/10.1016/S0014-5793(02)03746-8