Flavobacterium aquaticum sp. nov., a member of the Bacteroidetes isolated from a freshwater reservoir.

A novel bacterial strain, designated ARSA-111, was isolated from a freshwater reservoir in Cheonan, Korea. Phylogenetic analysis based on 16S rRNA gene sequences suggested that the isolate belonged to the genus Flavobacterium of phylum Bacteroidetes. The 16S rRNA gene sequence of strain ARSA-111 showed a high degree of sequence similarity to those of Flavobacteium cheonanense KACC 14972 (97.3%), F. aquatile JCM 20475 (97.1%), and other type strains of the genus Flavobacterium (< 97.0%). The phylogenetic tree and network analysis (i.e. median-joining) based on 16S rRNA gene sequences showed that strain ARSA-111 is most closely related to F. aquatile JCM 20475. DNA-DNA hybridization experiment revealed 70% of genomic relatedness among strain ARSA-111, F. aquatile JCM 20475 and F. cheonanense KACC 14972. The isolate had iso-C, iso-C, and iso-C 3-OH as predominant cellular fatty acids and MK-6 as a predominant menaquinone. The genomic DNA G+C content of the isolate was 35.6 mol%. On the basis of these data, strain ARSA-111 is considered to be a novel species of the genus Flavobacterium, for which the name Flavobacterium aquaticum sp. nov. is proposed. The type strain is strain ARSA-111 (=KACC 14973 =KCTC 23185 = JCM 17070). [ABSTRACT FROM AUTHOR]