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Leukemic cells with 11q23 translocations express granulocyte colony-stimulating factor (G-CSF) receptor and their proliferation is stimulated with G-CSF.
- Source :
- Leukemia (08876924); Mar98, Vol. 12 Issue 3, p382-389, 8p
- Publication Year :
- 1998
-
Abstract
- We report a 20-month-old boy with acute lymphoblastic leukemia with the 11q23 translocation whose blasts markedly increased in peripheral blood after intravenous granulocyte colony-stimulating factor (G-CSF) administration, but disappeared after stopping G-CSF. The in vitro study showed that the leukemic cells separated from this patient expressed G-CSF receptor (G-CSFR) and an addition of G-CSF stimulated their proliferation by 3H-thymidine incorporation assay (stimulation index, 4.9). To clarify whether or not leukemic cells with 11q23 translocations generally express G-CSFR and show proliferative response to G-CSF, we performed the similar in vitro experiments using eight leukemic cell lines with 11q23 translocations. We found that all cell lines examined expressed G-CSFR (20-98%) and proliferation of seven leukemic cell lines was significantly enhanced in response to G-CSF (stimulation index >1.5 in five cell lines), suggesting a possible participation of the G-CSF/G-CSFR interaction in the process of growth regulation of leukemic cells with 11q23 translocations. [ABSTRACT FROM AUTHOR]
- Subjects :
- LYMPHOBLASTIC leukemia
CHROMOSOMAL translocation
GRANULOCYTE-colony stimulating factor
ANTINEOPLASTIC agents
LYMPHOBLASTIC leukemia treatment
B cells
CELL division
CELL lines
CELL receptors
CHROMOSOMES
DNA
ETOPOSIDE
GENE mapping
GRANULOCYTES
IMMUNOPHENOTYPING
CHRONIC myeloid leukemia
CYTARABINE
MITOXANTRONE
CANCER cell culture
BLOOD
THERAPEUTICS
Subjects
Details
- Language :
- English
- ISSN :
- 08876924
- Volume :
- 12
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Leukemia (08876924)
- Publication Type :
- Academic Journal
- Accession number :
- 8883539
- Full Text :
- https://doi.org/10.1038/sj.leu.2400951