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Genipin stimulates glucose transport in C2C12 myotubes via an IRS-1 and calcium-dependent mechanism.
- Source :
- Journal of Endocrinology; Mar2013, Vol. 216 Issue 3, p353-362, 10p
- Publication Year :
- 2013
-
Abstract
- Genipin, a compound derived from Gardenia jasminoides Ellis fruits, has been used over the years in traditional Chinese medicine to treat symptoms of type 2 diabetes. However, the molecular basis for its antidiabetic effect has not been fully revealed. In this study, we investigated the effects of genipin on glucose uptake and signaling pathways in C<subscript>2</subscript>C<subscript>12</subscript> myotubes. Our study demonstrates that genipin stimulated glucose uptake in a time- and dose-dependent manner. The maximal effect was achieved at 2 h with a concentration of 10 μM. In myotubes, genipin promoted glucose transporter 4 (GLUT4) translocation to the cell surface, which was observed by analyzing their distribution in subcellular membrane fraction, and increased the phosphorylation of insulin receptor substrate-1 (IRS-1), AKT, and GSK3β. Meanwhile, genipin increased ATP levels, closed K<subscript>ATP</subscript> channels, and then increased the concentration of calcium in the cytoplasm in C<subscript>2</subscript>C<subscript>12</subscript> myotubes. Genipin-stimulated glucose uptake could be blocked by both the PI3-K inhibitor wortmannin and calcium chelator EGTA. Moreover, genipin increases the level of reactive oxygen species and ATP in C<subscript>2</subscript>C<subscript>12</subscript> myotubes. These results suggest that genipin activates IRS-1, PI3-K, and downstream signaling pathway and increases concentrations of calcium, resulting in GLUT4 translocation and glucose uptake increase in C<subscript>2</subscript>C<subscript>12</subscript> myotubes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00220795
- Volume :
- 216
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Journal of Endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 88946342
- Full Text :
- https://doi.org/10.1530/JOE-11-0473