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Novel Polyomaviruses of Nonhuman Primates: Genetic and Serological Predictors for the Existence of Multiple Unknown Polyomaviruses within the Human Population.

Authors :
Scuda, Nelly
Madinda, Nadege Freda
Akoua-Koffi, Chantal
Adjogoua, Edgard Valerie
Wevers, Diana
Hofmann, Jörg
Cameron, Kenneth N.
Leendertz, Siv Aina J.
Couacy-Hymann, Emmanuel
Robbins, Martha
Boesch, Christophe
Jarvis, Michael A.
Moens, Ugo
Mugisha, Lawrence
Calvignac-Spencer, Sébastien
Leendertz, Fabian H.
Ehlers, Bernhard
Source :
PLoS Pathogens; Jun2013, Vol. 9 Issue 6, p1-12, 12p
Publication Year :
2013

Abstract

Polyomaviruses are a family of small non-enveloped DNA viruses that encode oncogenes and have been associated, to greater or lesser extent, with human disease and cancer. Currently, twelve polyomaviruses are known to circulate within the human population. To further examine the diversity of human polyomaviruses, we have utilized a combinatorial approach comprised of initial degenerate primer-based PCR identification and phylogenetic analysis of nonhuman primate (NHP) polyomavirus species, followed by polyomavirus-specific serological analysis of human sera. Using this approach we identified twenty novel NHP polyomaviruses: nine in great apes (six in chimpanzees, two in gorillas and one in orangutan), five in Old World monkeys and six in New World monkeys. Phylogenetic analysis indicated that only four of the nine chimpanzee polyomaviruses (six novel and three previously identified) had known close human counterparts. To determine whether the remaining chimpanzee polyomaviruses had potential human counterparts, the major viral capsid proteins (VP1) of four chimpanzee polyomaviruses were expressed in E. coli for use as antigens in enzyme-linked immunoassay (ELISA). Human serum/plasma samples from both Côte d'Ivoire and Germany showed frequent seropositivity for the four viruses. Antibody pre-adsorption-based ELISA excluded the possibility that reactivities resulted from binding to known human polyomaviruses. Together, these results support the existence of additional polyomaviruses circulating within the human population that are genetically and serologically related to existing chimpanzee polyomaviruses. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
15537366
Volume :
9
Issue :
6
Database :
Complementary Index
Journal :
PLoS Pathogens
Publication Type :
Academic Journal
Accession number :
88957090
Full Text :
https://doi.org/10.1371/journal.ppat.1003429