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Circulating Levels of a Collagen Type V Propeptide Fragment in a Carbon Tetrachloride Reversible Model of Liver Fibrosis.
- Source :
- Biomarker Insights; 2012, Issue 7, p159-166, 8p
- Publication Year :
- 2012
-
Abstract
- Aim: To measure levels of the collagen V formation marker CO5-1230 during liver fibrosis progression and regression. Methods: Monoclonal antibodies were raised against the sequence TAALGDIMGH located at the start of the C-terminal propeptide between amino acid position 1230' and 1239' (CO5-1230). An assay developed using the biotin-streptavidin system was evaluated in a rat reversible model of fibrosis. Animals were treated for duration of 4, 6 and 8 weeks. Animals that were treated for 8 weeks were left to regress for a period of 14, 20 and 26 weeks. Results: Mean CO5-1230 level for control animals was found to be 8.7 ng/mL. CO5-1230 marker levels, at termination points, for CCl<subscript>4</subscript> treated animals was be 8.7 ng/mL at 4 weeks (P < 0.05, ROC: 0.83), 11.4 ng/mL at 6 weeks (P < 0.001, ROC: 0.93) and 10.8 ng/mL at 8 weeks (P < 0.05, ROC: 0.82). During regression phase, marker levels were statistically significantly decreased when compared with the marker levels at 8 weeks of treatment. Marker levels were found to be 5.9 ng/mL (P < 0.001, ROC: 0.8) after 14 weeks of regression, 3.9 ng/mL (P < 0.001, ROC: 0.95) after 20 weeks and 4.5 ng/mL (P < 0.001, ROC: 0.97) after 26 weeks of regression. Conclusions: The data indicates that CO5-1230 levels are statistically significantly increased when CCl<subscript>4</subscript> intoxication stimulus is applied in all treatment time points. CO5-1230 levels return back to control levels when the stimulus is removed. The above finding adds to our previous evaluation of the marker and suggests that CO5-1230 may be a promising potential marker for liver fibrosis staging and monitoring in both disease progression and regression. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 11772719
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Biomarker Insights
- Publication Type :
- Academic Journal
- Accession number :
- 89331677
- Full Text :
- https://doi.org/10.4137/BMI.S10975