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S100 A9 promotes human lung fibroblast cells activation through receptor for advanced glycation end-product-mediated extracellular-regulated kinase 1/2, mitogen-activated protein-kinase and nuclear factor-κ B-dependent pathways.
- Source :
- Clinical & Experimental Immunology; Sep2013, Vol. 173 Issue 3, p523-535, 13p, 1 Chart, 7 Graphs
- Publication Year :
- 2013
-
Abstract
- S100 A9 belongs to the S100 family of calcium-binding proteins and plays a key role in many inflammatory conditions. Recent studies have found that S100 A9 was elevated significantly in the bronchoalveolar lavage fluid of idiopathic pulmonary fibrosis patients, and might be a biomarker for fibrotic interstitial lung diseases. However, the exact function of S100 A9 in pulmonary fibrosis needs further studies. We performed this study to investigate the effect of S100 A9 on human embryo lung fibroblast ( HLF) proliferation and production of cytokines and collagen, providing new insights into the possible mechanism. S100 A9 promoted proliferation of fibroblasts and up-regulated expression of both proinflammatory cytokines interleukin ( IL)-6, IL-8, IL-1β and collagen type III. S100 A9 also induced HLF cells to produce α-smooth muscle actin (α- SMA) and receptor for advanced glycation end-product ( RAGE). In addition, S100 A9 caused a significant increase in extracellular-regulated kinase ( ERK)1/2 mitogen-activated protein kinase ( MAPK) phosphorylation, while the status of p38 and c-Jun N-terminal kinase ( JNK) phosphorylation remained unchanged. Treatment of cells with S100 A9 also enhanced nuclear factor kappa B ( NF-κ B) activation. RAGE blocking antibody pretreatment inhibited the S100 A9-induced cell proliferation, cytokine production and pathway phosphorylation. S100 A9-mediated cell activation was suppressed significantly by ERK1/2 MAPK inhibitor and NF-κ B inhibitor. In conclusion, S100 A9 promoted HLF cell growth and induced cells to secret proinflammatory cytokines and collagen through RAGE signalling and activation of ERK1/2 MAPK and NF-κ B pathways. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00099104
- Volume :
- 173
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Clinical & Experimental Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 89582971
- Full Text :
- https://doi.org/10.1111/cei.12139