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Bacterial community structure in cooling water and biofilm in an industrial recirculating cooling water system.
- Source :
- Water Science & Technology; 2013, Vol. 68 Issue 4, p940-947, 8p
- Publication Year :
- 2013
-
Abstract
- Microbial fouling is a serious problem in open recirculating cooling water systems. The bacterial communities that cause it have not been fully understood. In this study, we analyzed the community structure of free-living bacteria and particle-attached bacteria in cooling water, and bacteria in biofilm collected from the wall of the water reservoir in an industrial recirculating cooling water system by construction of a 16S rRNA gene clone library. Based on amplified ribosomal DNA restriction analysis, clones of all three libraries were clustered into 45 operational taxonomic units (OTUs). Thirteen OTUs displaying 91-96% sequence similarity to a type strain might be novel bacterial species. Noted differences in community structure were observed among the three libraries. The relative species richness of the free-living bacteria in cooling water was much lower than that of particle-attached bacteria and bacteria in biofilm. The majority of the free-living bacterial community (99.0%) was Betaproteobacteria. The predominant bacteria in the particle-attached bacterial community were Alphaproteobacteria (20.5%), Betaproteobacteria (27.8%) and Planctomycetes (42.0%), while those in the biofilm bacterial community were Alphaproteobacteria (47.9%), Betaproteobacteria (11.7%), Acidobacteria (13.1%) and Gemmatimonadetes (11.3%). To control microbial fouling in industrial recirculating cooling water systems, additional physiological and ecological studies of these species will be essential. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02731223
- Volume :
- 68
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Water Science & Technology
- Publication Type :
- Academic Journal
- Accession number :
- 90027550
- Full Text :
- https://doi.org/10.2166/wst.2013.334