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Overexpression of PLCE1 in Kazakh esophageal squamous cell carcinoma: implications in cancer metastasis and aggressiveness.
- Source :
- APMIS; Oct2013, Vol. 121 Issue 10, p908-918, 11p
- Publication Year :
- 2013
-
Abstract
- Three recent large-scale genome-wide association studies ( GWAS) in Chinese Han populations have identified an esophageal squamous cell carcinoma ( ESCC) susceptibility locus within phospholipase C epsilon 1 ( PLCE1) gene, which encodes a phospholipase involved in intracellular signaling. The expressed PLCE1 in ESCC, however, are inconsistent. This study examined PLCE1 expression by immunohistochemistry ( IHC) from 110 ethnic Kazakh ESCC patients and 50 from adjacent normal esophageal tissues ( NETs). The expressed PLCE1 was localized in cytoplasm, especially in the peripheral layers of cancer cell nests, which was significantly higher in tumors than in NETs (p < 0.001). Increased expression of PLCE1 was correlated with advanced tumor-node-metastasis (TNM) stages (p = 0.015) and lymph node metastasis (p = 0.003) in patients with ESCC. Of the 110 patients, we examined 50 paired ESCC tissues and corresponding NETs by quantitative RT-PCR (polymerase chain reaction) and the mean mRNA level of PLCE1 in ESCC was 1.85-fold higher compared with those in corresponding NETs (p = 0.0012). Meanwhile, 4 of 5 ESCC cell lines also showed elevated expression of PLCE1 mRNA. Furthermore, elevated expression of PLCE1 mRNA in Kazakh ESCC was associated with its immunoreactivity (ρ = 0.297, p = 0.040), lymph node metastasis (p < 0.001), and advanced TNM stages of ESCC (p = 0.013). To our knowledge, this study demonstrates for the first time that PLCE1 overexpression correlates with lymph node metastasis and advanced TNM stages of Kazakh ESCC, implicating a role of PLCE1 in cancer metastasis and aggressiveness in ethnic Kazakh patients with ESCC. Furthermore, the current findings may warrant investigations into whether inhibiting PLCE1 could be a strategy for targeted anticancer therapy particularly for Kazakh ESCC. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09034641
- Volume :
- 121
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- APMIS
- Publication Type :
- Academic Journal
- Accession number :
- 90469131
- Full Text :
- https://doi.org/10.1111/apm.12095