Isolating and Quantifying DNA in Mine, Coal Wastes, Soil Amendments and Artificial Soils for Mine Rehabilitation.

Mining is an activity that has been practiced by humans for years. Abandoned mines, tailings and spoils may have significant impact on the neighboring environments. The derelict mines and waste generated are the major issues of concern, as they are capable of discharging acid waters that are often saturated with iron, metals and metalloids. The process of rehabilitation is vital, which should include neutralizing the acid generating potential of the spoils and support vegetation. This research focuses on creating artificial soil for mine rehabilitation process by rejuvenating the mine waste with amendments, so that they support microbial growth and thus support vegetation in abandoned mines. Evidence for the growth of microorganisms and its support to vegetation is established by quantifying the DNA and conducting growth test with grass. [ABSTRACT FROM AUTHOR]