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Ethanol Exposure Potentiates fosB and junB Expression Induced by Muscarinic Receptor Stimulation in Neuroblastoma SH-SY5Y Cells.
- Source :
- Alcoholism: Clinical & Experimental Research; 1998, Vol. 22 Issue 1, p225-230, 6p
- Publication Year :
- 1998
-
Abstract
- Muscarinic receptor stimulation and activation of protein kinase C cause an increase in fosB and junB transcripts in human neuroblastoma SH-SY5Y cells. In this study, the effect of long-term ethanol exposure on these events was investigated. Carbachol-stimulated fosB and junB expression was elevated in ethanol-exposed cells compared with control cells. The potentiation was time- and dose-dependent on ethanol. Preincubation with muscarinic antagonists or protein kinase C inhibitor demonstrated that the carbachol-stimulated increase in fosB and junB mRNA levels was primarily mediated via M1 receptors and dependent on the activity of protein kinase C in both control and ethanol-exposed cells. Long-term ethanol exposure did not influence the expression of fosB and junB induced by activation of protein kinase C with phorbol ester. These results demonstrate that the muscarinic receptor-stimulated fosB and junB expression is sensitive to ethanol exposure in SH-SY5Y cells, suggesting that these genes participate in the regulation of neuronal function in response to chronic ethanol treatment. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- Volume :
- 22
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Alcoholism: Clinical & Experimental Research
- Publication Type :
- Academic Journal
- Accession number :
- 91183003
- Full Text :
- https://doi.org/10.1111/j.1530-0277.1998.tb03642.x