RP-HPLC SEPARATION AND STATISTICAL DATA PROCESSING OF DIFFERENT BATCHES OF AERIAL PARTS OF JOLOGBO.

This study was designed to develop a sensitive and reliable RP-HPLC method for the separation of aerial parts ofJologbo. Different extraction methods were used and compared to obtain the maximum number of constituents. Different chromatographic conditions were optimized to achieve optimum separation. A total of 98 peaks were obtained in the optimized separation method. Seasonal variation and difference in chemical composition among aerial parts of the plant were evaluated. Significant difference in the chemical composition was observed and statistical data processing supported the results. The correlation coefficient and vector cosine were calculated by chemometric analysis to evaluate peak retention times, peak areas, and peak heights between various batches (using selected peaks) which showed good repeatability. Gallic and protocatechuic acids were identified from the plant and their intra-day and inter-day repeatability values were calculated and convincing results were obtained. [ABSTRACT FROM AUTHOR]