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Effect of matrix metalloproteinase on autolysis of sea cucumber Stichopus japonicus.
- Source :
- Food Science & Biotechnology; Oct2013, Vol. 22 Issue 5, p1-3, 3p
- Publication Year :
- 2013
-
Abstract
- To investigate the relationship between matrix metalloproteinase (MMPs) and autolysis of sea cucumber Stichopus japonicus, the dermis homogenate was incubated at 25°C to induce autolysis. EDTA Na and 1,10-phenanthroline were used to verify the effect of MMPs on autolysis, which was measured by soluble protein and protein pattern. Soluble protein level increased during a 6-h autolysis process. SDS-PAGE demonstrated obvious protein degradation with the concomitant occurrence of degradation products. The above two indicators could be inhibited significantly by EDTA Na and 1,10-phenanthroline, indicating that MMPs might play a significant role in autolysis of sea cucumber. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 12267708
- Volume :
- 22
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Food Science & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 91587178
- Full Text :
- https://doi.org/10.1007/s10068-013-0210-x