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Differential IFN-γ production by adult and neonatal blood CD56+ natural killer ( NK) and NK-like- T cells in response to Trypanosoma cruzi and IL-15.
- Source :
- Parasite Immunology; Jan2014, Vol. 36 Issue 1, p43-52, 10p
- Publication Year :
- 2014
-
Abstract
- Early interferon-gamma ( IFN-γ) release by innate cells is critical to direct type 1 immune response able to control intracellular pathogens like Trypanosoma cruzi. Although CD56<superscript>bright</superscript> natural killer ( NK) cells are reported to be potent early IFN-γ producers, other CD56<superscript>+</superscript> cells like CD56<superscript>dim</superscript> NK cells and NK-like T cells have recently been shown to also release IFN-γ. We have here studied the contribution of each CD56<superscript>+</superscript> lymphocyte populations in early IFN-γ production in both adults and neonates. On this purpose, we analysed the kinetics of IFN-γ production by RT- PCR, ELISA and flow cytometry from 2 h onwards after T. cruzi and IL-15 stimulation and sought for the responding CD56<superscript>+</superscript> cells. CD56<superscript>bright</superscript> and CD56<superscript>dim</superscript> CD16<superscript>−</superscript> NK cells were the more potent IFN-γ early producers in response to IL-15 and parasites in adults and neonates. In both age groups, the majority of IFN-γ producing cells were NK cells. However, on the contrary to neonates, CD3<superscript>+</superscript> CD56<superscript>+</superscript> NK-like T cells and CD3<superscript>+</superscript> CD56<superscript>−</superscript> 'classical' T cells also contributed to early IFN-γ production in adults. Altogether, our results support that whereas NK cells responded almost similarly in neonates and adults, cord blood innate CD56<superscript>+</superscript> and CD56<superscript>−</superscript> T cells displayed major quantitative and qualitative defects that could contribute to the well-known neonatal immune immaturity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01419838
- Volume :
- 36
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Parasite Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 92764999
- Full Text :
- https://doi.org/10.1111/pim.12077