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Differential CD4+ T-cell responses of allergic and non-allergic subjects to the immunodominant epitope region of the horse major allergen Equ c 1.
- Source :
- Immunology; Jan2014, Vol. 141 Issue 1, p52-60, 9p
- Publication Year :
- 2014
-
Abstract
- The responses of allergen-specific CD4<superscript>+</superscript> T cells of allergic and healthy individuals are still incompletely understood. Our objective was to investigate the functional and phenotypic properties of CD4<superscript>+</superscript> T cells of horse-allergic and healthy subjects specific to the immunodominant epitope region of the major horse allergen Equ c 1. Specific T-cell lines (TCLs) and clones were generated from peripheral blood mononuclear cells with Equ c 1<subscript>143-160</subscript>, the peptide containing the immunodominant epitope region of Equ c 1. The frequency, proliferative response, cytokine production and HLA restriction of the cells were examined. The frequency of Equ c 1-specific CD4<superscript>+</superscript> T cells was low (approximately 1 per 10<superscript>6</superscript> CD4<superscript>+</superscript> T cells) in both allergic and non-allergic subjects. The cells of allergic subjects had a stronger proliferative capacity than those of non-allergic subjects, and they predominantly emerged from the memory T-cell pool and expressed the T helper type 2 cytokine profile, whereas the cells of non-allergic subjects emerged from the naive T-cell pool and produced low levels of interferon-γ and interleukin-10. T-cell response to Equ c 1<subscript>143-160</subscript> was restricted by several common HLA class II molecules from both DQ and DR loci. As the phenotypic and functional properties of Equ c 1-specific CD4<superscript>+</superscript> T cells differ between allergic and non-allergic subjects, allergen-specific T cells appear to be tightly implicated in the development of diseased or healthy outcome. Restriction of the specific CD4<superscript>+</superscript> T-cell response by multiple HLA alleles suggests that Equ c 1<subscript>143-160</subscript> is a promising candidate for peptide-based immunotherapy. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 141
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 92775478
- Full Text :
- https://doi.org/10.1111/imm.12166