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Evaluation of cellular effects of silicon dioxide nanoparticles.
- Source :
- Toxicology Mechanisms & Methods; Mar2014, Vol. 24 Issue 3, p196-203, 8p, 2 Charts, 6 Graphs
- Publication Year :
- 2014
-
Abstract
- Silica nanoparticles (nSiO<subscript>2</subscript>s) are an important type of manufactured nanoparticles. Although there are some reports about the cytotoxicity of nSiO<subscript>2</subscript>, the association between physical and chemical properties of nSiO<subscript>2</subscript>s and their cellular effects is still unclear. In this study, we examined the correlation between the physiochemical properties and cellular effects of three kinds of amorphous nSiO<subscript>2</subscript>s; sub-micro-scale amorphous SiO<subscript>2</subscript>, and micro-scale amorphous and crystalline SiO<subscript>2</subscript> particles. The SiO<subscript>2</subscript> particles were dispersed in culture medium and applied to HaCaT human keratinocytes and A549 human lung carcinoma cells. nSiO<subscript>2</subscript>s showed stronger protein adsorption than larger SiO<subscript>2</subscript> particles. Moreover, the cellular effects of SiO<subscript>2</subscript> particles were independent of the particle size and crystalline phase. The extent of cell membrane damage and intracellular ROS levels were different among nSiO<subscript>2</subscript>s. Upon exposure to nSiO<subscript>2</subscript>s, some cells released lactate dehydrogenase (LDH), whereas another nSiO<subscript>2</subscript> did not induce LDH release. nSiO<subscript>2</subscript>s caused a slight increase in intracellular ROS levels. These cellular effects were independent of the specific surface area and primary particle size of the nSiO<subscript>2</subscript>s. Additionally, association of solubility and protein adsorption ability of nSiO<subscript>2</subscript> to its cellular effects seemed to be small. Taken together, our data suggest that nSiO<subscript>2</subscript>s do not exert potent cytotoxic effects on cells in culture, especially compared to the effects of micro-scale SiO<subscript>2</subscript> particles. Further studies are needed to address the role of surface properties of nSiO<subscript>2</subscript>s on cellular processes and cytotoxicity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15376516
- Volume :
- 24
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Toxicology Mechanisms & Methods
- Publication Type :
- Academic Journal
- Accession number :
- 94643327
- Full Text :
- https://doi.org/10.3109/15376516.2013.879505