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Single-Cell States in the Estrogen Response of Breast Cancer Cell Lines.

Authors :
Casale, Francesco Paolo
Giurato, Giorgio
Nassa, Giovanni
Armond, Jonathan W.
Oates, Chris J.
CorĂ¡, Davide
Gamba, Andrea
Mukherjee, Sach
Weisz, Alessandro
Nicodemi, Mario
Source :
PLoS ONE; Feb2014, Vol. 9 Issue 2, p1-11, 11p
Publication Year :
2014

Abstract

Estrogen responsive breast cancer cell lines have been extensively studied to characterize transcriptional patterns in hormone-responsive tumors. Nevertheless, due to current technological limitations, genome-wide studies have typically been limited to population averaged data. Here we obtain, for the first time, a characterization at the single-cell level of the states and expression signatures of a hormone-starved MCF-7 cell system responding to estrogen. To do so, we employ a recently proposed model that allows for dissecting single-cell states from time-course microarray data. We show that within 32 hours following stimulation, MCF-7 cells traverse, most likely, six states, with a faster early response followed by a progressive deceleration. We also derive the genome-wide transcriptional profiles of such single-cell states and their functional characterization. Our results support a scenario where estrogen promotes cell cycle progression by controlling multiple, sequential regulatory steps, whose single-cell events are here identified. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
19326203
Volume :
9
Issue :
2
Database :
Complementary Index
Journal :
PLoS ONE
Publication Type :
Academic Journal
Accession number :
94730201
Full Text :
https://doi.org/10.1371/journal.pone.0088485