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Using optical profilometry to characterize cell membrane roughness influenced by amyloid-beta 42 aggregates and electric fields.
- Source :
- Journal of Biomedical Optics; Jan2014, Vol. 19 Issue 1, p1-5, 5p
- Publication Year :
- 2014
-
Abstract
- The membrane roughness of Neuro-2a neroblastoma cells is measured by using noninterferometric widefield optical profilometry. The cells are treated with the fibril and oligomer conformers of amyloid-beta (Aβ) 42, which is a peptide of 42 amino acids related to the development of Alzheimer's disease. We find that both the A β42 fibrils and Aβ42 oligomers reduced the cell membrane roughness, but the effect of Aβ42 oligomers was faster and stronger than that of the fibrils. We also apply direct-current electric field (dcEF) stimulations on the cells. A dcEF of 300 mV/mm can increase the membrane roughness under the treatment of Aβ42. These results suggest that Aβ42 can decrease the membrane compliance of live neuroblastoma cells, and dcEFs may counteract this effect. [ABSTRACT FROM AUTHOR]
- Subjects :
- ALZHEIMER'S disease research
INTERFEROMETRY
OLIGOMERS
CELL membranes
NEUROBLASTOMA
Subjects
Details
- Language :
- English
- ISSN :
- 10833668
- Volume :
- 19
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Biomedical Optics
- Publication Type :
- Academic Journal
- Accession number :
- 94830084
- Full Text :
- https://doi.org/10.1117/1.JBO.19.1.011009