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Direct electrochemistry of horseradish peroxidase based on hierarchical porous calcium phosphate microspheres.
- Source :
- Microchimica Acta; Apr2014, Vol. 181 Issue 5/6, p511-518, 8p
- Publication Year :
- 2014
-
Abstract
- Biomorphic calcium phosphate (CaP) microspheres with hierarchical porous structure were synthesized using natural cole pollen grains as templates and were further employed for the immobilization of horseradish peroxidase (HRP). Scanning electron microscopy and Fourier transform infrared spectroscopy revealed (a) the porous structure of the CaP microspheres, (b) the effective immobilization, and (c) the retention of the conformation of HRP on CaP. The immobilized HRP was placed on a glassy carbon electrode where it underwent a direct, fully reversible, and surface-controlled redox reaction with an electron transfer rate constant of 1.96 s. It also exhibits high sensitivity to the reduction of HO The response to HO is linear in the 5.00 nM to 1.27 μM concentration range, and the sensitivity is 30357 μA⋅mM⋅cm. The detection limit (at an SNR of 3) is as low as 1.30 nM. The apparent Michaelis-Menten constant ( K) of the immobilized enzyme is 0.92 μM. This new CaP with hierarchical porous structure therefore represents a material that can significantly promote the direct electron transfer between HRP and an electrode, and is quite attractive with respect to the construction of biosensors. [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 181
- Issue :
- 5/6
- Database :
- Complementary Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 95123689
- Full Text :
- https://doi.org/10.1007/s00604-013-1134-0