High Concentrations of Methyl Fluoride Affect the Bacterial Community in a Thermophilic Methanogenic Sludge.

To precisely control the application of methyl fluoride (CH₃F) for analysis of methanogenic pathways, the influence of 0–10% CH₃F on bacterial and archaeal communities in a thermophilic methanogenic sludge was investigated. The results suggested that CH₃F acts specifically on acetoclastic methanogenesis. The inhibitory effect stabilized at an initial concentration of 3–5%, with around 90% of the total methanogenic activity being suppressed, and a characteristic of hydrogenotrophic pathway in isotope fractionation was demonstrated under this condition. However, extended exposure (12 days) to high concentrations of CH₃F (>3%) altered the bacterial community structure significantly, resulting in increased diversity and decreased evenness, which can be related to acetate oxidation and CH₃F degradation. Bacterial clone library analysis showed that syntrophic acetate oxidizing bacteria Thermacetogenium phaeum were highly enriched under the suppression of 10% CH₃F. However, the methanogenic community did not change obviously. Thus, excessive usage of CH₃F over the long term can change the composition of the bacterial community. Therefore, data from studies involving the use of CH₃F as an acetoclast inhibitor should be interpreted with care. Conversely, CH₃F has been suggested as a factor to stimulate the enrichment of syntrophic acetate oxidizing bacteria. [ABSTRACT FROM AUTHOR]