Back to Search
Start Over
Modulation of the Expression of the Proinflammatory IL-8 Gene in Cystic Fibrosis Cells by Extracts Deriving from Olive Mill Waste Water.
- Source :
- Evidence-based Complementary & Alternative Medicine (eCAM); 2013, Vol. 2013, p1-11, 11p, 2 Diagrams, 6 Graphs
- Publication Year :
- 2013
-
Abstract
- A persistent recruitment of neutrophils in the bronchi of cystic fibrosis (CF) patients contributes to aggravate the airway tissue damage, suggesting the importance of modulating the expression of chemokines, including IL-8 during the management of the CF patients. Polyphenols rich extracts derived from waste water from olive mill, obtained by a molecular imprinting approach, have been investigated in order to discover compounds able to reduce IL-8 expression in human bronchial epithelial cells (IB3-1 cells), derived from a CF patient with a AF508/W1282X mutant genotype and stimulated with TNF-alpha. Initially, electrophoretic mobility shift assays (EMSAs) were performed to determine whether the different active principles were able to inhibit the binding between transcription factor (TF) NF-kappaB and DNA consensus sequences. Among different representative active principles present in the extract, three compounds were selected, apigenin, oleuropein, and cyanidin chloride, which displayed remarkable activity in inhibiting NF-kappaB/DNA complexes. Utilizing TNF-alpha-treated IB3-1 cells as experimental model system, we demonstrated that apigenin and cyanidin chloride are able to modulate the expression of the NF-kappaB-regulated IL-8 gene, while oleuropein showed no effect in regulating the expression of the gene IL-8. [ABSTRACT FROM AUTHOR]
- Subjects :
- CYSTIC fibrosis
PHYTOTHERAPY
OLIVE
ACADEMIC medical centers
ANALYSIS of variance
CELL culture
GENE expression
GENES
HIGH performance liquid chromatography
INFLAMMATION
INTERLEUKINS
MASS spectrometry
GENETIC mutation
POLYMERASE chain reaction
RESEARCH funding
T-test (Statistics)
GENOMICS
REVERSE transcriptase polymerase chain reaction
DATA analysis software
IN vitro studies
PREVENTION
THERAPEUTICS
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Volume :
- 2013
- Database :
- Complementary Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 95495228
- Full Text :
- https://doi.org/10.1155/2013/960603