Template-switching anchored polymerase chain reaction reliably amplifies functional lambda light chain transcripts of malignant lymphoma.

The article discusses research which was conducted to investigate whether template-switching anchored polymerase chain reaction (PCR) reliably amplifies functional lambda light chain transcripts of malignant lymphoma. Researchers used an alternative approach to current strategies for analysis of immunoglobulin transcripts that did not rely on the use of multiplex PCR to amplify the rearranged variable regions of antigen receptor loci. They found that their strategy was able to amplify the light chain transcripts of malignant lymphoma.