Realgar induces apoptosis in the chronic lymphocytic leukemia cell line MEC-1.

The aim of the present study was to investigate the effect of realgar on the viability, proliferation and apoptosis in the human chronic lymphocytic leukemia (CLL) cell line, MEC-1. Potential mechanisms mediating the effect were also explored in the experiment. Cultured MEC-1 cells were incubated with various concentrations of realgar for 24, 48 and 72 h. A WST-8 assay was employed to evaluate the effect on cell viability. Inhibitory effects on cell proliferation were determined using a 5-bromodeoxyuridine cell proliferation ELISA. The apoptotic effect on MEC-1 cells was evaluated by annexin V-fluorescein isothiocyanate/propidium iodide dual staining, followed by flow cytometry. Quantitative polymerase chain reaction was performed to determine the mRNA expression levels of BCL2-associated X protein (BAX), BCL2-like 1 (Bcl-xL), v-myc myelocytomatosis viral oncogene homolog (avian; c-Myc) and cyclin-dependent kinase inhibitor 1A (p21). It was found that viability and proliferation were significantly reduced while apoptotic rates increased in MEC-1 cells following exposure to realgar. Furthermore, mRNA expression of BAX and c-Myc was upregulated and downregulated, respectively, in realgar-treated MEC-1 cells. In conclusion, the results showed that realgar inhibits viability and proliferation and induces apoptosis of MEC-1 cells in a dose- and time-dependent manner. The effect may depend on the mitochondrial apoptosis pathway. The results of the present study may be beneficial in the identification of a new target therapy for CLL. [ABSTRACT FROM AUTHOR]