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Development of an in vitro model to measure bioactivity of botulinum neurotoxin A in rat bladder muscle strips.
- Source :
- BMC Urology; 2014, Vol. 14 Issue 1, p1-16, 16p
- Publication Year :
- 2014
-
Abstract
- Background Botulinum toxin A (BoNT-A) is a new treatment modality in various causes of bladder dysfunction; like neurogenic detrusor overactivity and overactive bladder. The best technique of administrating BoNT-A in patients is unknown. A validated in vitro model could be used to investigate newer intravesical administration techniques of BoNT-A. In this study, we describe the development and validation of in vitro model to measure inhibitory effects of BoNT-A on bladder strip contractions. Methods Rat bladder strips were mounted in organ baths filled with Krebs' solution. The strips were stimulated chemically (80 mM potassium chloride, 1 µM carbachol) and electrically (Electrical Field Stimulation (EFS) 100 shocks, 50 V, 20 Hz, every 3 minutes). The viability of the strips was measured by carbachol stimulation at the beginning and at the end of the experiments. The strips were incubated in various concentrations of BoNT-A (0.03, 0.2, 0.3 nM). Controls were incubated in Krebs' solution only. The inhibition of strip contraction induced by EFS was measured. These measurements were statistically analyzed with a loglogistic model representing diffusion. Results All strips remained viable during the experiments. Inhibition of strip contraction was observed after incubation with 0.3 nM BoNT-A. The measurements fitted to a log-logistic model describing diffusion of BoNT-A in the bladder strip. The parameters of the log-logistic model representing diffusion were significant for 0.3 nM BoNT-A. Incubation with 0.2 nM BoNT-A showed insignificant results for 2 out of 3 runs. Incubation with 0.03 nM BoNT-A did not result in significant inhibition of strip contractions. Conclusions An in vitro model was developed and validated in which the inhibitory effect of low concentrations of BoNT-A on bladder strip contractions can be measured. [ABSTRACT FROM AUTHOR]
- Subjects :
- BOTULINUM toxin
BLADDER diseases
LABORATORY rats
IN vitro studies
EXPERIMENTS
Subjects
Details
- Language :
- English
- ISSN :
- 14712490
- Volume :
- 14
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Urology
- Publication Type :
- Academic Journal
- Accession number :
- 96046912
- Full Text :
- https://doi.org/10.1186/1471-2490-14-37