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Sclerostin monoclonal antibody enhanced bone fracture healing in an open osteotomy model in rats.
- Source :
- Journal of Orthopaedic Research; Aug2014, Vol. 32 Issue 8, p997-1005, 9p
- Publication Year :
- 2014
-
Abstract
- ABSTRACT Sclerostin is a negative regulator of bone formation. Sclerostin monoclonal antibody (Scl-Ab) treatment promoted bone healing in various animal models. To further evaluate the healing efficiency of Scl-Ab in osteotomy healing, we investigated the time course effects of systemic administration of Scl-Ab on fracture repair in rat femoral osteotomy model. A total of 120 six-month-old male SD rats were subjected to transverse osteotomy at the right femur mid-shaft. Rats were treated with vehicle or Scl-Ab treatment for 3, 6, or 9 weeks. Fracture healing was evaluated by radiography, micro-CT, micro-CT based angiography, 4-point bending mechanical test and histological assessment. Scl-Ab treatment resulted in significantly higher total mineralized callus volume fraction, BMD and enhanced neovascularization. Histologically, Scl-Ab treatment resulted in a significant reduction in fracture callus cartilage at week 6 and increase in bone volume at week 9, associated with a greater proportion of newly formed bone area at week 6 and 9 by fluorescence microscopy. Mechanical testing showed significantly higher ultimate load in Scl-Ab treatment group at week 6 and 9. This study has demonstrated that Scl-Ab treatment enhanced bone healing in a rat femoral osteotomy model, as reflected in increased bone formation, bone mass and bone strength. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:997-1005, 2014. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 07360266
- Volume :
- 32
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Journal of Orthopaedic Research
- Publication Type :
- Academic Journal
- Accession number :
- 96716810
- Full Text :
- https://doi.org/10.1002/jor.22636