OPTIMIZATION OF PCR THROUGH MANIPULATION OF CYCLE TIMES AND INCLUSION OF FORMAMIDE.

Polymerase chain reaction (PCR) is an in vitro technique to produce million fold copies of a particular segment of DNA. PCR should optimally yield a unique product unless designed otherwise. Artefacts appear many times, which affect the success of downstream applications. Prevention of non specific amplification using formamide and manipulation of cycling time is discussed in this article. [ABSTRACT FROM AUTHOR]