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Superior In Vitro Stimulation of Human CD8+ T-Cells by Whole Virus versus Split Virus Influenza Vaccines.
- Source :
- PLoS ONE; Jul2014, Vol. 9 Issue 7, p1-12, 12p
- Publication Year :
- 2014
-
Abstract
- Pandemic and seasonal influenza viruses cause considerable morbidity and mortality in the general human population. Protection from severe disease may result from vaccines that activate antigen-presenting DC for effective stimulation of influenza-specific memory T cells. Special attention is paid to vaccine-induced CD8<superscript>+</superscript> T-cell responses, because they are mainly directed against conserved internal influenza proteins thereby presumably mediating cross-protection against circulating seasonal as well as emerging pandemic virus strains. Our study showed that influenza whole virus vaccines of major seasonal A and B strains activated DC more efficiently than those of pandemic swine-origin H1N1 and pandemic-like avian H5N1 strains. In contrast, influenza split virus vaccines had a low ability to activate DC, regardless which strain was investigated. We also observed that whole virus vaccines stimulated virus-specific CD8<superscript>+</superscript> memory T cells much stronger compared to split virus counterparts, whereas both vaccine formats activated CD4<superscript>+</superscript> Th cell responses similarly. Moreover, our data showed that whole virus vaccine material is delivered into the cytosolic pathway of DC for effective activation of virus-specific CD8<superscript>+</superscript> T cells. We conclude that vaccines against seasonal and pandemic (-like) influenza strains that aim to stimulate cross-reacting CD8<superscript>+</superscript> T cells should include whole virus rather than split virus formulations. [ABSTRACT FROM AUTHOR]
- Subjects :
- CD8 antigen
T cells
INFLUENZA vaccines
IN vitro studies
PANDEMICS
LEUCOCYTES
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 97362252
- Full Text :
- https://doi.org/10.1371/journal.pone.0103392