Back to Search
Start Over
Replication competent virus as an important source of bias in HIV latency models utilizing single round viral constru.
- Source :
- Retrovirology; 2014, Vol. 11 Issue 1, p1-9, 9p
- Publication Year :
- 2014
-
Abstract
- The central memory T cell (TCM) model forms a unique HIV-1 latency model based on primary cells that closely resemble in vivo TCM. The virus employed in this model is based on an engineered vector incapable of replication after initial infection. We show that despite this strategy, replication competent viral particles are released into the culture medium due to recombination between overlapping sequences of the env deleted HIV genome that is cotransfected with intact env. This finding emphasizes the need for careful data analysis and interpretation if similar constructs are employed and urges for additional caution during laboratory work. [ABSTRACT FROM AUTHOR]
- Subjects :
- HIV infections
GENOMES
DATA analysis
RECOMBINATION (Chemistry)
HIV
Subjects
Details
- Language :
- English
- ISSN :
- 17424690
- Volume :
- 11
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Retrovirology
- Publication Type :
- Academic Journal
- Accession number :
- 97923223
- Full Text :
- https://doi.org/10.1186/s12977-014-0070-3