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Functional characterization of human CD34+ cells that express low or high levels of the membrane antigen CD111 (nectin 1).
- Source :
- Leukemia (08876924); Jun2003, Vol. 17 Issue 6, p1137, 9p
- Publication Year :
- 2003
-
Abstract
- Nectins are recently described adhesion molecules that are widely expressed on many tissues, including the hematopoietic tissue. Nectin 1 (CD111) is expressed on a higher proportion of mobilized peripheral blood (mPB) than cord blood (CB) CD34[SUP+] cells, and of CD34[SUP+]/CD38[SUP+] cells when compared with CD34[SUP+]/CD38[SUP-] cells. We studied functional properties of human CB and mPB CD34[SUP+] cells that express low or high levels of CD111. CD34[SUP+]/CD111[SUPdim] cells contain a higher proportion of cells in G0/G1 phase than CD34[SUP+]/CD111[SUPbright] cells. CD34[SUP+]/CD111[SUPbright] cells contain more erythroid progenitors: CFU-E, than their counterparts, which on the opposite contain more HPP-CFC. Limiting dilution analyses demonstrate a higher frequency of immature progenitors: cobblestone-area colony-forming cells, in CD34[SUP+]/CD111[SUPdim] than in CD34[SUP+]/CD111[SUPbright] cells. In vitro differentiation assays demonstrate a higher frequency of B-, T- and dendritic-cell precursors, but less NK-cell precursors in CD34[SUP+]/CD111[SUPdim] cells. Evaluation of engraftment in NOD-SCID mice shows that SCID repopulating cells are more frequent among mPB CD34[SUP+]/CD111[SUPdim] cells. Liquid culture of CD34[SUP+]/CD111[SUPdim] cells with erythropoietin shows that CD111 expression increases simultaneously with CD36, following CD71 and before glycophorin A expression. In conclusion, immature human hematopoietic progenitors express low levels of CD111 on their surface. During erythroid differentiation CD34[SUP+] cells acquire higher levels of the CD111 antigen. [ABSTRACT FROM AUTHOR]
- Subjects :
- CELL adhesion molecules
CORD blood
Subjects
Details
- Language :
- English
- ISSN :
- 08876924
- Volume :
- 17
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Leukemia (08876924)
- Publication Type :
- Academic Journal
- Accession number :
- 9851953
- Full Text :
- https://doi.org/10.1038/sj.leu.2402916