Back to Search
Start Over
CD4+ T Cells Expressing Latency-Associated Peptide and Foxp3 Are an Activated Subgroup of Regulatory T Cells Enriched in Patients with Colorectal Cancer.
- Source :
- PLoS ONE; Sep2014, Vol. 9 Issue 9, p1-9, 9p
- Publication Year :
- 2014
-
Abstract
- Latency-associated peptide (LAP) - expressing regulatory T cells (Tregs) are important for immunological self-tolerance and immune homeostasis. In order to investigate the role of LAP in human CD4<superscript>+</superscript>Foxp3<superscript>+</superscript> Tregs, we designed a cross-sectional study that involved 42 colorectal cancer (CRC) patients. The phenotypes, cytokine-release patterns, and suppressive ability of Tregs isolated from peripheral blood and tumor tissues were analyzed. We found that the population of LAP-positive CD4<superscript>+</superscript>Foxp3<superscript>+</superscript> Tregs significantly increased in peripheral blood and cancer tissues of CRC patients as compared to that in the peripheral blood and tissues of healthy subjects. Both LAP<superscript>+</superscript> and LAP<superscript>−</superscript> Tregs had a similar effector/memory phenotype. However, LAP<superscript>+</superscript> Tregs expressed more effector molecules, including tumor necrosis factor receptor II, granzyme B, perforin, Ki67, and CCR5, than their LAP<superscript>−</superscript> negative counterparts. The in vitro immunosuppressive activity of LAP<superscript>+</superscript> Tregs, exerted via a transforming growth factor-β–mediated mechanism, was more potent than that of LAP<superscript>−</superscript> Tregs. Furthermore, the enrichment of LAP<superscript>+</superscript> Treg population in peripheral blood mononuclear cells (PBMCs) of CRC patients correlated with cancer metastases. In conclusion, we found that LAP<superscript>+</superscript> Foxp3<superscript>+</superscript> CD4<superscript>+</superscript> Treg cells represented an activated subgroup of Tregs having more potent regulatory activity in CRC patients. The increased frequency of LAP<superscript>+</superscript> Tregs in PBMCs of CRC patients suggests their potential role in controlling immune response to cancer and presents LAP as a marker of tumor-specific Tregs in CRC patients. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 98617972
- Full Text :
- https://doi.org/10.1371/journal.pone.0108554