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The expression sequence tag is an effective method for screening DNA segments that predict urinary bladder transitional cell carcinoma prognosis.

Authors :
Pei-Shan Yang
Yu-Chao Hsu
Yu-Hsiang Lin
Cheng-Pang Hou
Chien-Lun Chen
Phei-Lang Chang
Horng-Heng Juang
Ke-Hung Tsui
Source :
OncoTargets & Therapy; 2014, Vol. 7, p1777-1781, 5p
Publication Year :
2014

Abstract

Purpose: We validated the use of expression sequence tags (ESTs) as an effective method of screening for DNA segments that could predict urothelial cell carcinoma and for identifying ESTs with such predictive value. Patients and methods: From 2004 to 2009, eleven patients were enrolled in this study: six with high-grade bladder carcinoma and five with low-grade bladder carcinoma. ESTs were used to screen for differential gene expression in a high-grade cell line (MGH-U1) and in a premalignant cell line (MGH-U4). Immunohistochemistry and real-time reverse transcriptionpolymerase chain reaction were used to validate the degree of EST expression and the prognostic value of ESTs. Results: Apoferritin H subunit (FTH1 protein) exhibited increased expression in high-grade bladder carcinoma compared with that seen in low-grade carcinoma. Immunohistochemistry and real-time reverse transcription-polymerase chain reaction both supported the higher expression of FTH1 in high-grade urothelial carcinoma. Conclusion: ESTs are useful for detecting the FTH1 protein, which predicts the prognosis of patients with bladder carcinoma. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
11786930
Volume :
7
Database :
Complementary Index
Journal :
OncoTargets & Therapy
Publication Type :
Academic Journal
Accession number :
98656789
Full Text :
https://doi.org/10.2147/OTT.S69239