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The in vivo J-difference editing MEGA-PRESS technique for the detection of n-3 fatty acids.

Authors :
Škoch, Antonín
Tošner, Zdeněk
Hájek, Milan
Source :
NMR in Biomedicine; Nov2014, Vol. 27 Issue 11, p1293-1299, 7p
Publication Year :
2014

Abstract

In this study, we present a method for the detection of n-3 fatty acid (n-3 FA) signals using MRS in adipose tissue in vivo. This method (called oMEGA-PRESS) is based on the selective detection of the CH<subscript>3</subscript> signal of n-3 FA using the MEGA-PRESS (MEshcher-GArwood Point-RESolved Spectroscopy) J-difference editing technique. We optimized the envelope shape and frequency of spectral editing pulses to minimize the spurious co-editing and incomplete subtraction of the CH<subscript>3</subscript> signal of other FAs, which normally obscure the n-3 FA CH<subscript>3</subscript> signal in MR spectra acquired using standard PRESS techniques. The post-processing of the individual data scans with the phase and frequency correction before data subtraction and averaging was implemented to further improve the quality of in vivo spectra. The technique was optimized in vitro on lipid phantoms using various concentrations of n-3 FA and examined in vivo at 3 T on 15 healthy volunteers. The proportion of n-3 FA estimated by the oMEGA-PRESS method in phantoms showed a highly significant linear correlation with the n-3 FA content determined by gas chromatography. The signal attributed to n-3 FA was observed in all subjects. Comparisons with the standard PRESS technique revealed an enhanced identification of the n-3 FA signal using oMEGA-PRESS. The presented method may be useful for the non-invasive quantification of n-3 FA in adipose tissue, and could aid in obtaining a better understanding of various aspects of n-3 FA metabolism. Copyright © 2014 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09523480
Volume :
27
Issue :
11
Database :
Complementary Index
Journal :
NMR in Biomedicine
Publication Type :
Academic Journal
Accession number :
98922759
Full Text :
https://doi.org/10.1002/nbm.3189