Rapid analysis and identification for the alkaloids from Ranunculus japonicus by UPLC/Q-TOF-MS.

To establish a rapid and effective method for analysis and identification of the alkaloids from Ranunculus japonicus Thunb by ultra-performance liquid chromatogaraphy with quadruple-time-of-flight mass spectrometry (UPLC/Q-TOP-MS) and discuss their fragmentation regularity, the UPLC/Q-TOF-MS was used to identify the alkaloids from Ranunculus japonicus Thunb by their MS data, tandem characteristic fragment ions and standards. In the end. 12 alkaloids were identified from Ranunculus japonicus for the first time, and their fragmentation regularity was discussed. Thus, a rapid and effective analysis and identification method for the alkaloids from Ranunculus japonicus by UPLC/Q-TOF-MS is established. [ABSTRACT FROM AUTHOR]