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Optimization of a Natural Medium for Cellulase by a Marine Aspergillus niger Using Response Surface Methodology.
- Source :
- Applied Biochemistry & Biotechnology; Aug2012, Vol. 167 Issue 7, p1963-1972, 10p
- Publication Year :
- 2012
-
Abstract
- The components of a natural medium were optimized to produce cellulase from a marine Aspergillus niger under solid state fermentation conditions by response surface methodology. Eichhornia crassipes and natural seawater were used as a major substrate and a source of mineral salts, respectively. Mineral salts of natural seawater could increase cellulase production. Raw corn cob and raw rice straw showed a significant positive effect on cellulase production. The optimum natural medium consisted of 76.9 % E. crassipes (w/w), 8.9 % raw corn cob (w/w), 3.5 % raw rice straw (w/w), 10.7 % raw wheat bran (w/w), and natural seawater (2.33 times the weight of the dry substrates). Incubation for 96 h in the natural medium increased the biomass to the maximum. The cellulase production was 17.80 U/g the dry weight of substrates after incubation for 144 h. The natural medium avoided supplying chemicals and pretreating substrates. It is promising for future practical fermentation of environment-friendly producing cellulase. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02732289
- Volume :
- 167
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Applied Biochemistry & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 99372676
- Full Text :
- https://doi.org/10.1007/s12010-012-9734-4