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Reversible Ca2+ Switch of An Engineered Allosteric Antioxidant Selenoenzyme.
- Source :
- Angewandte Chemie International Edition; Dec2014, Vol. 53 Issue 49, p13536-13539, 4p
- Publication Year :
- 2014
-
Abstract
- A Ca<superscript>2+</superscript>-responsive artificial selenoenzyme was constructed by computational design and engineering of recoverin with the active center of glutathione peroxidase (GPx). By combining the recognition capacity for the glutathione (GSH) substrate and the steric orientation of the catalytic selenium moiety, the engineered selenium-containing recoverin exhibits high GPx activity for the catalyzed reduction of H<subscript>2</subscript>O<subscript>2</subscript> by glutathione (GSH). Moreover, the engineered selenoenzyme can be switched on/off by Ca<superscript>2+</superscript>-induced allosterism of the protein recoverin. This artificial selenoenzyme also displays excellent antioxidant ability when it was evaluated using a mitochondrial oxidative damage model, showing great potential for controlled catalysis in biomedical applications. [ABSTRACT FROM AUTHOR]
- Subjects :
- ANTIOXIDANTS
GLUTATHIONE peroxidase
SELENIUM
CATALYSIS
GLUTATHIONE
OXIDATION
Subjects
Details
- Language :
- English
- ISSN :
- 14337851
- Volume :
- 53
- Issue :
- 49
- Database :
- Complementary Index
- Journal :
- Angewandte Chemie International Edition
- Publication Type :
- Academic Journal
- Accession number :
- 99708178
- Full Text :
- https://doi.org/10.1002/anie.201407135